Integrating Whole Cell Biotransformation of Aroma Compounds into a Novel Biorefinery Concept

The synthesis of aroma compounds that are utilized as precursors of multiple synthesis chains in the pharmaceutical industries and as ingredients in food and fragrance industries can be carried out using chemical processes, enzyme biocatalysis and whole cell biotransformation. Whole cell biotransformation has the potential of being more environmentally benign than chemical synthesis and more cost-effective as compared to enzyme catalysis. In a recently published study by the authors, the aroma compound Ethyl(3)hydroxybutyrate was produced by whole cell biotransformation under aerobic and anaerobic conditions. The yield of the anaerobic processes was similar to that of the aerobic processes, but additionally generated CO2 and ethanol as useful by-products. In this chapter we illustrate how the production process of Ethyl(3)hydroxybutyrate by whole cell biotransformation can be integrated into a novel biorefinery concept, based on the finding that the production of Ethyl(3)hydroxybutyrate under anaerobic conditions is efficient and environmentally friendly. CO2 may be converted to bio-methane together with H2 produced from excess regenerative power. A life cycle assessment confirmed that the anaerobic whole cell biotransformation process embedded into a biorefinery concept including bio-methane production has a lower environmental impact as compared to a concept based on the aerobic whole cell biotransformation

A Review of Double-Walled and Triple-Walled Carbon Nanotube Synthesis and Applications

Double- and triple-walled carbon nanotubes (DWNTs and TWNTs) consist of coaxially-nested two and three single-walled carbon nanotubes (SWNTs). They act as the geometrical bridge between SWNTs and multi-walled carbon nanotubes (MWNTs), providing an ideal model for studying the coupling interactions between different shells in MWNTs. Within this context, this article comprehensively reviews various synthetic routes of DWNTs’ and TWNTs’ production, such as arc discharge, catalytic chemical vapor deposition and thermal annealing of pea pods (i.e., SWNTs encapsulating fullerenes). Their structural features, as well as promising applications and future perspectives are also discussed. Keywords: carbon nanotubes; double-walled carbon nanotubes; triple-walled carbon nanotubes; synthesis; catalytic chemical vapor deposition; arc discharge; fullerenes; pea pod

Predictors for target vessel failure after recanalization of chronic total occlusions in patients undergoing surveillance coronary angiography

(1) Background: Knowledge about predictors for the long-time patency of recanalized chronic total coronary occlusions (CTOs) is limited. Evidence from invasive follow-up in the absence of acute coronary syndrome (routine surveillance coronary angiography) is scarce. (2) Methods: In a monocentric-retrospective analysis, we obtained baseline as well as periprocedural data of patients undergoing routine invasive follow-up. We defined target vessel failure (TVF) as a combined primary endpoint, consisting of re-occlusion, restenosis, and target vessel revascularization (TVR). (3) Results: We included 93 consecutive patients (15.1% female) from October 2013 to May 2018. After a follow-up period of 206 ± 129 days (median 185 (IQR 127–237)), re-occlusion had occurred in 7.5%, restenosis in 11.8%, and TVR in 5.4%; the cumulative incidence of TVF was 15.1%. Reduced TIMI-flow immediately after recanalization (OR for TVR: 11.0 (95% CI: 2.7–45.5), p = 0.001) as well as female gender (OR for TVR: 11.0 (95% CI: 2.1–58.5), p = 0.005) were found to be predictive for pathological angiographic findings at follow-up. Furthermore, higher blood values of high-sensitive troponin after successful revascularization were associated with all endpoints. Interestingly, neither the J-CTO score nor the presence of symptoms at the follow-up visit could be correlated to adverse angiographic results. (4) Conclusions: In this medium-sized cohort of patients with surveillance coronary angiography, we were able to identify reduced TIMI flow and female gender as the strongest predictors for future TVF. Keywords: chronic total occlusion; target vessel failure; re-occlusion; surveillance coronary angiograph

G′ band in double- and triple-walled carbon nanotubes: A Raman study

Double- and triple-walled carbon nanotubes are studied in detail by laser energy-dependent Raman spectroscopy in order to get a deeper understanding about the second-order G[superscript '] band Raman process, general nanotube properties, such as electronic and vibrational properties, and the growth method itself. In this work, the inner nanotubes from the double- and triple-walled carbon nanotubes are produced through the encapsulation of fullerene peapods with high-temperature thermal treatments. We find that the spectral features of the G[superscript '] band, such as the intensity, frequency, linewidth, and line shape are highly sensitive to the annealing temperature variations. We also discuss the triple-peak structure of the G[superscript '] band observed in an individual triple-walled carbon nanotube taken at several laser energies connecting its Raman spectra with that for the G[superscript '] band spectra obtained for bundled triple-walled carbon nanotubes.National Science Foundation (U.S.) (Grant 1004147

Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo

Objective: Gut homing of lymphocytes via adhesion molecules has recently emerged as new target for therapy in inflammatory bowel diseases. We aimed to analyze the in vivo homing of effector (Teff) and regulatory (Treg) T cells to the inflamed gut via α4β7 and GPR15. Design: We assessed the expression of homing receptors on T cells in peripheral blood and inflamed mucosa. We studied the migration pattern and homing of Teff and Treg cells to the inflamed gut using intravital confocal microscopy and FACS in a humanized mouse model in DSS-treated NSG (NOD.Cg-Prkdcscid-Il2rgtm1Wjl/SzJ) mice. Results: Expression of GPR15 and α4β7 was significantly increased on Treg rather than Teff cells in peripheral blood of patients with ulcerative colitis (UC) as compared to Crohn´s disease and controls. In vivo analysis in a humanized mouse model showed augmented gut homing of UC Treg cells as compared to controls. Moreover, suppression of UC (but not control) Teff and Treg cell homing was noted upon treatment with the α4β7 antibody vedolizumab. In contrast, siRNA blockade of GPR15 had only effects on homing of Teff cells but did not affect Treg homing in UC. Clinical vedolizumab treatment was associated with marked expansion of UC Treg cells in peripheral blood. Conclusion: α4β7 rather than GPR15 is crucial for increased colonic homing of UC Treg cells in vivo, while both receptors control UC Teff homing. Vedolizumab treatment impairs homing of UC Treg cells leading to their accumulation in peripheral blood with subsequent suppression of systemic effector T cell expansion

Accretion onto black holes formed by direct collapse

One possible scenario for the formation of massive black holes (BHs) in the early Universe is from the direct collapse of primordial gas in atomic-cooling dark matter haloes in which the gas is unable to cool efficiently via molecular transitions. We study the formation of such BHs, as well as the accretion of gas onto these objects and the high energy radiation emitted in the accretion process, by carrying out cosmological radiation hydrodynamics simulations. In the absence of radiative feedback, we find an upper limit to the accretion rate onto the central object which forms from the initial collapse of hot (~ 10^4 K) gas of the order of 0.1 MSun per year. This is high enough for the formation of a supermassive star, the immediate precursor of a BH, with a mass of the order of 10^5 MSun. Assuming that a fraction of this mass goes into a BH, we track the subsequent accretion of gas onto the BH self-consistently with the high energy radiation emitted from the accretion disk. Using a ray-tracing algorithm to follow the propagation of ionizing radiation, we model in detail the evolution of the photoionized region which forms around the accreting BH. We find that BHs with masses of the order of 10^4 MSun initially accrete at close to the Eddington limit, but that the accretion rate drops to of order 1 percent of the Eddington limit after ~ 10^6 yr, due to the expansion of the gas near the BH in response to strong photoheating and radiation pressure. One signature of the accretion of gas onto BHs formed by direct collapse, as opposed to massive Pop III star formation, is an extremely high ratio of the luminosity emitted in He II 1640 to that emitted in H_alpha (or Ly_alpha); this could be detected by the James Webb Space Telescope. Finally, we briefly discuss implications for the coevolution of BHs and their host galaxies.Comment: 16 pages; 17 figures, slightly reduced quality; MNRAS in pres

An empirical prediction for stellar metallicity distributions in nearby galaxies

We combine star-formation histories derived from observations of high redshift galaxies with measurements of the z~0 relation between gas-phase metallicity, stellar mass, and star formation rate to make an explicit and completely empirical connection between near-field and distant galaxy observations. Our approach relies on two basic assumptions: 1) galaxies' average paths through time in stellar mass vs. star formation rate space are represented by a family of smooth functions that are determined by the galaxies' final stellar mass, and 2) galaxies grow and become enriched with heavy elements such that they always evolve along the mass--metallicity--star formation rate relation. By integrating over these paths, we can track the chemical evolution of stars in galaxies in a model independent way, without the need for explicit assumptions about gas inflow, outflow, or star formation efficiency. Using this approach, we present predictions of stellar metallicity (i.e., O/H) distribution functions for present day star-forming galaxies of different stellar masses and the evolution of the alpha-element stellar metallicity-mass relation since z~1. The metallicity distribution functions are fairly well described as Gaussians, truncated at high metallicity, with power-law tails to low metallicity. We find that the stellar metallicity distribution for Milky Way mass galaxies is in reasonable agreement with observations for our Galaxy, and that the predicted stellar mass vs. mean stellar metallicity relation at z=0 agrees quite well with results derived from galaxy surveys. This validates the assumptions that are implicit in our simple approach. Upcoming observations will further test these assumptions and their range of validity, by measuring the mean stellar mass-metallicity relation up to z~1, and by measuring the stellar metallicity distributions over a range of galaxy masses.Comment: 8 pages, 2 figures, 2 tables. MNRAS, in pres